REDACS: Regional emergency-driven adaptive cluster sampling for effective COVID-19 management

As COVID-19 is spreading, national agencies need to monitor and track several metrics. Since we do not have perfect testing programs on the hand, one needs to develop an advanced sampling strategies for prevalence study, control and management. Here we introduce REDACS: Regional emergency-driven adaptive cluster sampling for effective COVID-19 management and control and justify its usage for COVID-19. We show its advantages over classical massive individual testing sampling plans. We also point out how regional and spatial heterogeneity underlines proper sampling. Fundamental importance of adaptive control parameters from emergency health stations and medical frontline is outlined. Since the Northern hemisphere entered Autumn and Winter season (this paper was originally submitted in November 2020), practical illustration from spatial heterogeneity of Chile (Southern hemisphere, which already experienced COVID-19 winter outbreak peak) is underlying the importance of proper regional heterogeneity of sampling plan. We explain the regional heterogeneity by microbiological backgrounds and link it to behavior of Lyapunov exponents. We also discuss screening by antigen tests from the perspective of "on the fly" biomarker validation, i.e., during the screening. [ FROM AUTHOR] Copyright of Stochastic Analysis & Applications is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

Development and validation of scores on a measure to appraise student-teacher relationships in a remote learning environment: Validation of the virtual learning assessment

The purpose of this dissertation was to validate scores for a new survey tool, The Virtual Learning Assessment. This 53-item measure was designed with the intent that if scores are validated it could provide a means in which student-teacher relationships and the attributes that enhance those relationships in a remote setting could be assessed. To test the validity of the this newly designed measure 532 Middle School students were asked to rate the level of impact specific teacher actions had on middle school student's relationship with a teacher while in a remote learning environment. This survey was developed during the COVID-19 pandemic, and the survey was distributed electronically during students first semester back in face-to-face learning. Results revealed a 2-factor solution, these two factors were named, teachers' support for students' social-emotional well-being (factor 1) and personalized learning for students (factor 2). Confirmatory Factor Analysis revealed an acceptable-to questionable fit, with the data based on the guidelines provided by Dimitrov (2012) and Schreiber et al. (2006), CMIN, chi2 (323) = 770.01, p < .001, chi2/df = 2.38;CFI = .88;RMSEA = .07, 90% CI (.06, .07);and SRMR = .05. In addition, the 2-factor solution indicated a Normative Fit Index (NFI = .81), Tucker-Lewis Index (TLI = .87). Values for this statistic range between 0 and 1 with Bentler and Bonnet (1980) recommending values greater than .90 indicating a good fit (Hooper & Mullen, 2008). The preliminary attempt to validate scores on the Virtual Learning Assessment suggests that while a true latent structure does exist amongst the data set with a 2-factor solution, further refinement of the survey items is necessary before further use of the measure in conducting research. (PsycInfo Database Record (c) 2023 APA, all rights reserved)

Assessment of the clinical and analytical performance of the Aptima SARS-CoV-2 assay using the VALCOR protocol.

BACKGROUND: The COVID-19 pandemic highlighted the importance of diagnostic testing against curbing the spread of SARS-CoV-2. The urgent need and scale for diagnostic tools resulted in manufacturers of SARS-CoV-2 assays receiving emergency authorization that lacked robust analytical or clinical evaluation. As it is highly likely that testing for SARS-CoV-2 will continue to play a central role in public health, the performance characteristics of assays should be evaluated to ensure reliable diagnostic outcomes are achieved. METHODS: VALCOR or "VALidation of SARS-CORona Virus-2 assays" is a study protocol designed to set up a framework for test validation of SARS-CoV-2 virus assays. Using clinical samples collated from VALCOR, the performance of Aptima SARS-CoV-2 assay was assessed against a standard comparator assay. Diagnostic test parameters such as sensitivity, specificity and overall per cent agreement were calculated for the clinical performance of Aptima SARS-CoV-2 assay. RESULTS: A total of 180 clinical samples were tested with an addition of 40 diluted clinical specimens to determine the limit of detection. When compared to the standard comparator assay Aptima had a sensitivity of 100.0% [95% CI 95.9-100.0] and specificity of 96.7% [95% CI 90.8-99.3]. The overall percent agreement was 98.3% with an excellent Cohen's coefficient of κ = 0.967 [95% CI 0.929-1.000]. For the limit of detection, Aptima was able to detect all of the diluted clinical samples. CONCLUSION: In conclusion. validation of Aptima SARS-CoV-2 assay using clinical samples collated through the VALCOR protocol showed excellent test performance. Additionally, Aptima demonstrated high analytical sensitivity by detecting all diluted clinical samples corresponding to a low limit of detection.

Development and validation of scores on a measure to appraise student-teacher relationships in a remote learning environment: Validation of the virtual learning assessment

The purpose of this dissertation was to validate scores for a new survey tool, The Virtual Learning Assessment. This 53-item measure was designed with the intent that if scores are validated it could provide a means in which student-teacher relationships and the attributes that enhance those relationships in a remote setting could be assessed. To test the validity of the this newly designed measure 532 Middle School students were asked to rate the level of impact specific teacher actions had on middle school student's relationship with a teacher while in a remote learning environment. This survey was developed during the COVID-19 pandemic, and the survey was distributed electronically during students first semester back in face-to-face learning. Results revealed a 2-factor solution, these two factors were named, teachers' support for students' social-emotional well-being (factor 1) and personalized learning for students (factor 2). Confirmatory Factor Analysis revealed an acceptable-to questionable fit, with the data based on the guidelines provided by Dimitrov (2012) and Schreiber et al. (2006), CMIN, chi2 (323) = 770.01, p < .001, chi2/df = 2.38;CFI = .88;RMSEA = .07, 90% CI (.06, .07);and SRMR = .05. In addition, the 2-factor solution indicated a Normative Fit Index (NFI = .81), Tucker-Lewis Index (TLI = .87). Values for this statistic range between 0 and 1 with Bentler and Bonnet (1980) recommending values greater than .90 indicating a good fit (Hooper & Mullen, 2008). The preliminary attempt to validate scores on the Virtual Learning Assessment suggests that while a true latent structure does exist amongst the data set with a 2-factor solution, further refinement of the survey items is necessary before further use of the measure in conducting research. (PsycInfo Database Record (c) 2023 APA, all rights reserved)

The psychometric properties and the factorial structure of COVID-19 Vaccines Acceptance scale (VAC-COVID-19) within the Arabic language in a Palestinian contex.

BACKGROUND: The COVID-19 Vaccines Acceptance scale (VAC-COVID-19) is an international measure designed to evaluate vaccination acceptance against the COVID-19 virus. The current scale was translated from English to Arabic and validated within the Palestinian context. AIMS: Our study aimed to test the factorial structure and the psychotic properties of the VAC-COVID-19 within the Palestinian context using exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) through 484 participants selected using online method techniques. FINDINGS: The VAC-COVID-19 was a reliable and valid method in assessing COVID-19 vaccine acceptance among Palestinians. Results of CFA indicated a stable construct of a two-factor solution in assessing COVID-19 vaccination acceptance in a Palestinian context. (1) Reasons for not receiving the vaccination, and (2) for receiving the vaccination. CONCLUSION: The VAC-COVID-19 was a valid method to assess vaccination acceptance in the Arabic language within the Palestinian context. Therefore, it is recommended to conduct similar studies with diverse samples in Palestinian society; it would be prudent to target at-risk populations needed to develop the scale and its factorial structure. The VAC-COVID-19 can be a useful measure to assess vaccination acceptance among Palestinians, enabling health providers to implement interventions to modify negative attitudes toward not receiving vaccinations.

Psychometric properties of the COVID stress scales (CSS) within Arabic language in a Palestinian context.

The main goal of this study was to examine the psychometric properties of the COVID Stress Scales (CSS) in the Palestinian context and the factorial structure of the instrument. The CSS, a newly emerging internationally standardized measure of stress related to being exposed to or contracting COVID-19, was translated and validated for a Palestinian context to ensure that it can be used to measure COVID-19 stress. The sample of the study consisted of 860 Palestinian adults living in the West Bank of Palestine. Participants' age ranged from 20 to 48 years old (M = 34.7, SD =13.46). They were all recruited from online advertisements, e-mail campaigns, blogs, social media, and SMS campaigns. The CSS was found to be valid in the Arabic language within a Palestinian context. The confirmatory factor analysis yielded six factors: (1) Fears about the dangerousness of COVID-19, (2) fears about the personal social, and economic consequences of COVID-19, fears of disruption in the supply chain, fears of looting or rioting, (3) COVID-19-xenophobia, fears that foreigners are sources of COVID-19, (4) fears about sources of COVID-19-related contamination,(5) traumatic stress symptoms related to COVID-19, and (6) COVID-19-related checking which is consisting with the ordinal structure the scale. The CSS demonstrated a high level of validity and reliability in a Palestinian context and therefore can be considered for future studies as the COVID-19 pandemic persists. Further investigations using the Arabic Language of CSS may have far-reaching implications for measuring and combating the stress of COVID-19 at a personal and societal level for uniquely at-risk populations such as in the occupied territories of Palestine.

Psychometric Properties of the Fear of COVID-19 Scale (FCV-19S) in a Palestinian Context

This study translated and validated the Fear of COVID-19 Scale (FCV-19S) in a Palestinian context. The FCV-19S is a newly emerging internationally standardized measure of anxiety related to being exposed to or contracting COVID-19. This research begins to identify factors that affect this phenomenon including gender (with females having more fear than males), education (individuals without college degrees demonstrating higher levels of fear) and smoking status (with smokers demonstrating higher levels of fear than non-smokers). The FCV-19 confirmed a one-factor structure in assessing fear of COVID-19 in the Palestinian context. Moreover, the measure demonstrated high levels of validity and reliability in a Palestinian context and therefor can be considered for future studies as the COVID-19 pandemic persists. Further investigations using the Arabic Language of FCV-19S may have far-reaching implications for measuring and combating the fear of COVID-19 at a personal and societal level for uniquely at-risk populations such as in the occupied territories of Palestine. © 2022 Michigan Publishing. All rights reserved.

VALCOR: a protocol for the validation of SARS-corona virus-2 assays.

BACKGROUND: Testing for SARS-CoV-2, together with vaccination, is one of the most vital strategies in curbing the current COVID-19 pandemic. The pandemic has led to an unprecedented need for diagnostic testing and the rapid emergence of an abundance of commercial assays on the market. Due to the nature of the pandemic and in the interest of health protection, many of these assays received provisional authorisation for emergency use without thorough validation. To limit false negative and false positive results, it is key to define common criteria that SARS-CoV-2 assays need to fulfil. VALCOR or "VALidation of SARS-CORona Virus-2 assays" is a protocol designed to set up a framework for test validation of SARS-CoV-2 virus assays. OBJECTIVES: VALCOR is a study protocol for the validation of assays used for confirmation of the presence of SARS-CoV-2 in patients with COVID-19 disease or the screening of carriers of SARS-CoV-2 virus by the identification of viral RNA in oropharyngeal and/or nasopharyngeal specimens or other specimens from the human respiratory tract. METHODS: The VALCOR panel of samples will contain clinical human specimens and standardised artificial specimens. The collection of clinical specimens will include nasopharyngeal or oropharyngeal specimens or other specimens from the respiratory tract obtained from COVID-19 patients and healthy carriers of SARS-CoV-2 as well as specimens from subjects not carrying SARS-CoV-2. Artificial specimens include calibrated amounts of viral RNA of SARS-CoV-2 sequences provided by established competent agencies that produce reference materials for the assessment of the limit of detection of each assay. The panel of samples are sent from a central reference laboratory (having access to biobanks of clinical specimens tested already for SARS-CoV-2 with a reference comparator assay) to participating laboratories for testing with a SARS-CoV-2 index assay that requires evaluation. DISCUSSION: VALCOR provides a harmonised and standard framework to benchmark the testing performance of SARS-CoV-2 assays that are rapidly evolving. As the pandemic incited an urgent need for testing capacity, there is a gap in the comprehensive validation of SARS-CoV-2 assays. This study will generate comprehensive validation data for assays used for the diagnosis of SARS-CoV-2 and may serve as a basis for other validation protocols.

Sperm quality and absence of SARS-CoV-2 RNA in semen after COVID-19 infection: a prospective, observational study and validation of the SpermCOVID test.

OBJECTIVE: To study the contagiousness of sperm and its influence on fertility after recovery from COVID-19 infection. DESIGN: Prospective cohort study. SETTING: University medical center. PATIENT(S): One hundred twenty Belgian men who had recovered from proven COVID-19 infection. INTERVENTION(S): No intervention was performed. MAIN OUTCOME MEASURE(S): Semen quality was assessed using the World Health Organisation criteria. DNA damage to sperm cells was assessed by quantifying the DNA fragmentation index and the high density stainability. Finally antibodies against SARS-CoV2 spike-1 antigen, nuclear and S1-receptor binding domain were measured by Elisa and chemilumenscent microparticle immunoassays, respectively. RESULT(S): SARS-CoV-2 RNA was not detected in semen during the period shortly after infection nor at a later time. Mean progressive motility was reduced in 60% of men tested shortly (<1 month) after COVID-19 infection, 37% of men tested 1 to 2 months after COVID-19 infection, and 28% of men tested >2 months after COVID-19 infection. Mean sperm count was reduced in 37% of men tested shortly (<1 month) after COVID-19 infection, 29% of men tested 1 to 2 months after COVID-19 infection, and 6% of men tested >2 months after COVID-19 infection. The severity of COVID-19 infection and the presence of fever were not correlated with sperm characteristics, but there were strong correlations between sperm abnormalities and the titers of SARS-CoV-2 IgG antibody against spike 1 and the receptor- binding domain of spike 1, but not against nucleotide, in serum. High levels of antisperm antibodies developed in three men (2.5%). CONCLUSION(S): Semen is not infectious with SARS-CoV-2 at 1 week or more after COVID-19 infection (mean, 53 days). However, couples with a desire for pregnancy should be warned that sperm quality after COVID-19 infection can be suboptimal. The estimated recovery time is 3 months, but further follow-up studies are under way to confirm this and to determine if permanent damage occurred in a minority of men.